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For important safety information, refer to the safety booklet provided with the HTS option. 6 BD LSRFortessa Cell Analyzer User’s Guide z BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD™ High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. z BD FACSFlow Supply System User’s Guide: Describes to)running)an)HTS)experiment.)The)HTS)uses)more)sheath)fluid)than)loading) thetubes.) 2. Switch)the)acquisition)control)switch)to)plate)mode.) 3. Remove)the)tube)of)DI)water)fromthe)SIT.) 4. Attach)the)HTS)sample)coupler)to)the)cytometer)SIT.)Slide)the)sample)coupler) onto)the)SIT)until)you)reach)a)hard)stop.)Make)sure)the)sample)coupler) FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you Page 1 FACSFortessa – HTS User Instructions The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer provides rapid, fully automated sample acquisition from microtiter plates.

Bd fortessa hts user guide

➢ Switc 27 Oct 2004 The BD LSR II flow cytometer is equipped with safety features for your protection. Operate the instrument only as directed in the BD LSR II User's. Shutdown Procedure (printed version on back cover of User Guide Binder). 8. The 5 Laser LSR Fortessa (“5L”) is located in CNY149, room. 5227. The BD High Throughput Sampler (HTS) is a high-speed sample loading device used on.

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Use the Acquisition Dashboard to acquire and record well data. Run Plate runs the wells from the current position to … to using the BD™ High Throughput Sampler (HTS; Plate Loader) on the DHVI Flow Facility L01 and L02 bench-top analyzers. 2.0 Scope and Application This SOP applies specifically to the BD LSRII (L01/L02) instruments in the MSRBII Building, Hallway 2045, Bay 5 and 6. This procedure applies to Flow The BD™ High Throughput Sampler (HTS) option is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate.

High Throughput In Vitro Assessment of Latency Reversing

It also contains a description of BD FACSDiva software features specific to the HTS. † BD FACSFlow™ Supply System User’s Guide: Describes the optional automated sheath and waste fluid control system. 1. Switch on the LSR-Fortessa at the side of the instrument. This will warm up the lasers and pressurize the fluidics.

Flow Cytometry Unit – Biomedical Core facility.
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Locate and loosen the positioning screw that secures the HTS to the cytometer support bracket (Figure 4-17 on page 133). This Getting Started guide provides tutorials to familiarize you with BD FACSDiva™ software. If you are a new user, use the tutorials to get started with the software. If you are an experienced user, try the updated tutorials to become familiar with new software features. Updated features are highlighted in the New Features section. New Features University of Helsinki Performance.

Turn on the computer. Wait for the computer to boot up. 2. Log in to the computer (facsuser, password =BDIS) and open BD FACSDiva software. Log in to Diva at the prompt with your Diva username and password. 3. Turn on main power switch (Green button-right side) 4.
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It processes 96 samples under 15 minutes with less than 0.5% sample carryover. Cited in over 4,500 publications, the BD LSRFortessa is your next step to discovery. This 4.5 days course teaches the operation of the BD LSRFortessa, BD LSRFortessa X-20 and BD FACSCelesta. Additionally, the challenges of successful multi-parameter analysis in terms of antibody-fluorochrome selection, instrument setup including High Throughput Sampler (HTS) & Quality Control (BD™ 2018-12-11 · BD_LSR-Fortessa_18-COLOR_20181211 Cytometer: LSRFortessa Cytometer Name: LSRFortessa Serial Number: H64780000027 Input Device: HTS Window Extension: 10.00 User: Administrator Institution: N/A Software: BD FACSDiva 8.0.1 Date: 12/11/2018 2:59:48 PM Blue Laser (488nm) FSC Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential.

If you are an experienced user, try the updated tutorials to become familiar with new software features. Updated Service Maintenance Plan for BD LSR Fortessa SORP with HTS and BD Influx with Lasers Overview : Reference number: Registered Users To register interest in this opportunity or to electronically respond, you must first sign in. Click the Sign In button below. How to Identify Problems with Flow Cytometry Experiment Design – Bad Data Part 3 Two weeks ago I covered my red flags for identifying bad panel design (read that here if you missed it!). This week I’ll go over my red flags for experiment design, which mostly means controls.
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If you are a new user, use the tutorials to get started with the software. If you are an experienced user, try the updated tutorials to become familiar with new software features.

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Leave the support arm to the side.

Updated features are highlighted in the New Features section. New Features JF Fortessa HTS Laser Chart. M Canto Laser Chart JF Fortessa Laser Chart. Protocols. Cell Surface Staining Protocol. BD HTS User Guide Performance.